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room temperature rt  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology room temperature rt
    Room Temperature Rt, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/room+temperature+rt/ADAR1+Antibody/bio_rxiv__64898__2026__01__29__701210-280-19-29
    Average 95 stars, based on 207 article reviews
    room temperature rt - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Direct simulation of hypertensive stress on endothelial cells: a streamlined model of in-vitro-hypertension
    Article Snippet: Proteins (10 μg/lane) along with a molecular weight ladder (ThermoFisher Scientific, catalog number #SM1841) were fractionated by NuPAGE BIS-TRIS by 4%–12% SDS-polyacrylamide pre-cast gel electrophoresis (ThermoFisher Scientific, catalog number NP0321BOX) and transferred to nitrocellulose using iBlot 3 Transfer Stacks Nitrocellulose (ThermoFisher Scientific, catalog number IB33002). .. The membranes were blocked for 2 h at room temperature (RT) in PBS/0.05% Tween 20 (PT) (Cell Signaling Technology) containing 5% non-fat dry milk (Santa Cruz Biotechnology, Dallas, TX, United States) and incubated overnight at 4 °C with anti-human NFκB (1:1,000 in PT plus 5% BSA, Cell Signaling Technology, catalog number #8242) or anti-human phosphorylated NFκB (pNFκB, 1:1,000 in PT plus 5% BSA, Cell Signaling Technology, catalog number #3039) or anti-human p38MAPK (1:1,000 in PT plus 5% BSA, Cell Signaling Technology, catalog number #9212) or anti-human phosphorylated p38MAPK (pp38MAPK, 1:1,000 in PT plus 5% BSA, Cell Signaling Technology, catalog number #9211) After three washes, the membranes were incubated for 1 h at RT in PT/5% non-fat dry milk plus HRP-conjugated secondary antibodies (1:5000, Cell Signaling Technology, catalog number #7074) and developed using ECL (Westar Supernova, Cyanagen, Bologna, IT, catalog number XLS3,0100). .. Signals were detected using radiographic films (Kodak, Rochester, NY, United States).

    Article Title: Pathogenic role of acyl coenzyme A binding protein (ACBP) in Cushing's syndrome.
    Article Snippet: Two thousand H4 GFP–LC3 cells per well were seeded in 384-well μClear imaging plates (cat. no. 781091, Greiner Bio-One) 24 h before experimentation. .. Following compound treatment (Supplementary Table 1), cells were fixed with 4% paraformaldehyde containing 10 mM Hoechst 33342 (cat. no. H3570, Thermo Fisher Scientific) in phosphate-buffered saline (PBS) for 30 min at room temperature (RT), permeabilized with 0.1% Triton X-100 for 10 min and blocked with 5% bovine serum albumin (BSA) in PBS for 1–2 h, followed by overnight incubation with anti-ACBP antibody (cat. no. sc-376853, Santa Cruz Biotechnology) at 4 °C. .. After 2× washing with PBS, cells were incubated with AlexaFluor-conjugated secondary antibody (cat. no. A-11004; Thermo Fisher Scientific) for 2 h at RT.

    Article Title: Isolation and identification of lactic acid bacteria from ginseng sprouts and research on their probiotic, anti-inflammatory, and anti-cancer activity
    Article Snippet: Equal amounts of lysates were resolved using SDS-PAGE, and the separated proteins were transferred onto a nitrocellulose membrane (GE Healthcare Life Sciences, MA, USA). .. The membrane was blocked with 5% skim milk (BD Difco, NJ, USA) in TBST at room temperature (RT) for 30 min and then incubated with anti-cyclin D1 (Cat. no #sc-8396, Santa Cruz, Dallas, TX, USA), anti c-Myc (Cat. no #3198, Cell Signaling, Danvers, MA, USA), anti-GAPDH (Cat. no #sc-47724, Santa Cruz Biotechnology, Dallas, TX, USA), or anti-PARP (Cat. no #9542, Cell Signaling Technology, Danvers, MA, USA) antibodies at 4 °C overnight. .. The blots were then incubated with the corresponding horseradish peroxidase-conjugated secondary antibodies (anti-rabbit [Cat. no #RSA1221; BioActs, Incheon, Republic of Korea] or anti-mouse [#RSA1122; BioActs, Incheon, Republic of Korea]) at RT for 2 h. Band intensity was quantified using the ImageJ 1.53e software (National Institutes of Health, MD, USA).

    Article Title: The RNA Editome of Esophageal Squamous Cell Carcinoma Identifies an ADAR1-CDK13 Editing Feedback Loop Mediating cGAS–STING Activation in Early Tumorigenesis
    Article Snippet: .. The membrane was blocked with 5% non-fat milk powder in 1 × TBST (Biocomma, P164230701) for 1-2 hours at room temperature (RT), followed by incubation with primary anti-ADAR1 antibody (Santa Cruz, sc-73408; 1:200 dilution) overnight (∼16 h) in 4°C refrigerator. .. After three times washes with 1 × TBST (10 min), the membrane was incubated with secondary anti-mouse antibody (Abcam, ab205719; 1:2000 dilution) in 5% non-fat milk powder in 1 × TBST for 1-2 h at RT.

    Article Title: Pathogenic role of acyl coenzyme A binding protein (ACBP) in Cushing’s syndrome
    Article Snippet: Two thousand H4 GFP–LC3 cells per well were seeded in 384-well μClear imaging plates (cat. no. 781091, Greiner Bio-One) 24 h before experimentation. .. Following compound treatment (Supplementary Table ), cells were fixed with 4% paraformaldehyde containing 10 mM Hoechst 33342 (cat. no. H3570, Thermo Fisher Scientific) in phosphate-buffered saline (PBS) for 30 min at room temperature (RT), permeabilized with 0.1% Triton X-100 for 10 min and blocked with 5% bovine serum albumin (BSA) in PBS for 1–2 h, followed by overnight incubation with anti-ACBP antibody (cat. no. sc-376853, Santa Cruz Biotechnology) at 4 °C. .. After 2× washing with PBS, cells were incubated with AlexaFluor-conjugated secondary antibody (cat. no. A-11004; Thermo Fisher Scientific) for 2 h at RT.

    Article Title: Conditional Overexpression of Net1 Enhances the Trans-Differentiation of Lgr5 + Progenitors into Hair Cells in the Neonatal Mouse Cochlea.
    Article Snippet: Following the separation of protein samples by 10% PAGE, which was performed using the PAGE Gel Rapid Preparation Kit (PG112, Epizyme), the samples were transferred to a PVDF membrane (ISEQ00010, Millipore). .. The membranes were then blocked with 5% (vol/ vol) skimmed milk in 1× TBST (1× Tris buffer solution containing 0.1% Tween- 20) for 1 h at room temperature (RT) and incubated overnight at 4°C with Net1 antibody (sc- 271,941, Santa Cruz) or β- actin antibody (ab119716, Abcam). .. The membrane was finally incubated with peroxidase- conjugated goat anti- mouse IgG- HRP and goat anti- rabbit IgG- HRP antibodies (M21001; M21002, Abmart) for 1 h at RT after washing three times (10 min each time) with 1× TBST.

    Article Title: Direct simulation of hypertensive stress on endothelial cells: a streamlined model of in-vitro-hypertension
    Article Snippet: 3 Transfer Stacks Nitrocellulose (ThermoFisher Scientific, catalog number IB33002). .. The membranes were blocked for 2 h at room temperature (RT) in PBS/0.05% Tween 20 (PT) (Cell Signaling Technology) containing 5% non-fat dry milk (Santa Cruz Biotechnology, Dallas, TX, United States) and incubated overnight at 4 °C with anti-human NFκB (1:1,000 in PT plus 5% BSA, Cell Signaling Technology, catalog number #8242) or anti-human phosphorylated NFκB (pNFκB, 1:1,000 in PT plus 5% BSA, Cell Signaling Technology, catalog number #3039) or anti-human p38MAPK (1:1,000 in PT plus 5% BSA, Cell Signaling Technology, catalog number #9212) or antihuman phosphorylated p38MAPK (pp38MAPK, 1:1,000 in PT plus 5% BSA, Cell Signaling Technology, catalog number #9211) After three washes, the membranes were incubated for 1 h at RT in PT/5% non-fat dry milk plus HRP-conjugated secondary antibodies (1:5000, Cell Signaling Technology, catalog number #7074) and developed using ECL (Westar Supernova, Cyanagen, Bologna, IT, catalog number XLS3,0100). .. Signals were detected using radiographic films (Kodak, Rochester, NY, United States).

    Article Title: Prolonged survival by combination treatment with a standardized herbal extract from Japanese Kampo-medicine (Juzentaihoto) and gemcitabine in an orthotopic transplantation pancreatic cancer model
    Article Snippet: .. Subsequently, the cells were blocked in 0.5% bovine serum albumin (BSA) in PBS for 1 h at room temperature (RT) and incubated with the mouse monoclonal anti-vimentin antibody (Santa Cruz Biotechnology, E-5, 1:200) and the rabbit monoclonal anti-E-cadherin antibody (Cell signaling, 24E10, 1:100) in 0.5% BSA for 1 h at RT. .. After three washes with 0.1% Tween20 in PBS for 10 min each, the cells were incubated for 1 h at RT with fluorescence-labeled secondary antibodies: anti-mouse (Thermo Fisher, A-11003, Alexa Fluor 546) and anti-rabbit (Thermo Fisher, A-21206, Alexa Fluor 488), each diluted 1:500 in 0.5% BSA in PBS.

    Saline:

    Article Title: Pathogenic role of acyl coenzyme A binding protein (ACBP) in Cushing's syndrome.
    Article Snippet: Two thousand H4 GFP–LC3 cells per well were seeded in 384-well μClear imaging plates (cat. no. 781091, Greiner Bio-One) 24 h before experimentation. .. Following compound treatment (Supplementary Table 1), cells were fixed with 4% paraformaldehyde containing 10 mM Hoechst 33342 (cat. no. H3570, Thermo Fisher Scientific) in phosphate-buffered saline (PBS) for 30 min at room temperature (RT), permeabilized with 0.1% Triton X-100 for 10 min and blocked with 5% bovine serum albumin (BSA) in PBS for 1–2 h, followed by overnight incubation with anti-ACBP antibody (cat. no. sc-376853, Santa Cruz Biotechnology) at 4 °C. .. After 2× washing with PBS, cells were incubated with AlexaFluor-conjugated secondary antibody (cat. no. A-11004; Thermo Fisher Scientific) for 2 h at RT.

    Article Title: Pathogenic role of acyl coenzyme A binding protein (ACBP) in Cushing’s syndrome
    Article Snippet: Two thousand H4 GFP–LC3 cells per well were seeded in 384-well μClear imaging plates (cat. no. 781091, Greiner Bio-One) 24 h before experimentation. .. Following compound treatment (Supplementary Table ), cells were fixed with 4% paraformaldehyde containing 10 mM Hoechst 33342 (cat. no. H3570, Thermo Fisher Scientific) in phosphate-buffered saline (PBS) for 30 min at room temperature (RT), permeabilized with 0.1% Triton X-100 for 10 min and blocked with 5% bovine serum albumin (BSA) in PBS for 1–2 h, followed by overnight incubation with anti-ACBP antibody (cat. no. sc-376853, Santa Cruz Biotechnology) at 4 °C. .. After 2× washing with PBS, cells were incubated with AlexaFluor-conjugated secondary antibody (cat. no. A-11004; Thermo Fisher Scientific) for 2 h at RT.

    Membrane:

    Article Title: Isolation and identification of lactic acid bacteria from ginseng sprouts and research on their probiotic, anti-inflammatory, and anti-cancer activity
    Article Snippet: Equal amounts of lysates were resolved using SDS-PAGE, and the separated proteins were transferred onto a nitrocellulose membrane (GE Healthcare Life Sciences, MA, USA). .. The membrane was blocked with 5% skim milk (BD Difco, NJ, USA) in TBST at room temperature (RT) for 30 min and then incubated with anti-cyclin D1 (Cat. no #sc-8396, Santa Cruz, Dallas, TX, USA), anti c-Myc (Cat. no #3198, Cell Signaling, Danvers, MA, USA), anti-GAPDH (Cat. no #sc-47724, Santa Cruz Biotechnology, Dallas, TX, USA), or anti-PARP (Cat. no #9542, Cell Signaling Technology, Danvers, MA, USA) antibodies at 4 °C overnight. .. The blots were then incubated with the corresponding horseradish peroxidase-conjugated secondary antibodies (anti-rabbit [Cat. no #RSA1221; BioActs, Incheon, Republic of Korea] or anti-mouse [#RSA1122; BioActs, Incheon, Republic of Korea]) at RT for 2 h. Band intensity was quantified using the ImageJ 1.53e software (National Institutes of Health, MD, USA).

    Article Title: The RNA Editome of Esophageal Squamous Cell Carcinoma Identifies an ADAR1-CDK13 Editing Feedback Loop Mediating cGAS–STING Activation in Early Tumorigenesis
    Article Snippet: .. The membrane was blocked with 5% non-fat milk powder in 1 × TBST (Biocomma, P164230701) for 1-2 hours at room temperature (RT), followed by incubation with primary anti-ADAR1 antibody (Santa Cruz, sc-73408; 1:200 dilution) overnight (∼16 h) in 4°C refrigerator. .. After three times washes with 1 × TBST (10 min), the membrane was incubated with secondary anti-mouse antibody (Abcam, ab205719; 1:2000 dilution) in 5% non-fat milk powder in 1 × TBST for 1-2 h at RT.



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